Coding

Part:BBa_K1113501:Design

Designed by: Belen Cespedes   Group: iGEM13_UC_Chile   (2013-09-18)

GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2026
    Illegal AgeI site found at 2197
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2712


Design Notes

This part it constructed with a mutation in the Prefix (from “cggccgc” to “atacctg”) which erase the NotI restriction site, this modification allow the correct design of primers for Gibson assembly. This part its also constructed on the pSB4K5 standard vector


Source

The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley). All the rest are bricks available in the Registry

References